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KMID : 1157520220180020063
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International Journal of Clinical Preventive Dentistry
2022 Volume.18 No. 2 p.63 ~ p.69
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A Study on Hydrogen Peroxide Mediated Calcium Elution and Surface Change of Bovine Enamel
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Ahn Jae-Hyun
Jeong Yong-Beam
Kang Nae-Gyu
Im Song-Hee
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Abstract
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Objective: The purpose of this study is to investigate the calcium elution and surface change of bovine enamel due to the action of hydrogen peroxide.
Methods: Bovine enamels were treated at 37℃ for 3 hours by hydrogen peroxide solution (0%-10%), pH changed water (3.0-9.0) and commercial beverages. The eluted calcium amounts were analyzed with an inductively coupled plasma atomic emission spectrometer. Confidence interval estimates were analyzed for each eluted calcium amount with 95% confidence rate (α=0.05). After the separately prepared bovine enamel’s surfaces were exposed under same condition, they were evaluated by scanning electron microscope (SEM) and energy dispersion X-ray spectroscopy (EDS).
Results: The eluted calcium amount from bovine enamel according to the concentration of hydrogen peroxide was significantly different but very small (0.41-1.76 μg/ml). Although significant differences were observed between in the presence and absence of hydrogen peroxide, the eluted calcium amounts were very small at the pH 5.0 or higher. A large amount of calcium elution (37.29 μg/ml) and damage to the surface of bovine enamel were observed at the pH 3.0 around. The eluted calcium amounts of commercial beverages showing low pH were 4.13-65.69 μg/ml and surface damages of bovine enamel were larger than hydrogen peroxide solutions of pH 5.0 or higher.
Conclusion: Hydrogen peroxide solution with pH 5.0 or higher showed less calcium elution from bovine teeth than water with pH 3.0, carbonated beverages and orange juice, and did not cause big change of the surface of bovine teeth.
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KEYWORD
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bovine, calcium, hydrogen peroxide, tooth whitening
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